Sandwich Elisa Protocol Leinco Technologies

Sandwich Elisa Protocol Leinco Technologies An enzyme linked immunosorbent assay or sandwich elisa is a scientific technique used by researchers to detect the presence of an antibody or an antigen in a sample. an elisa can also be used as a diagnostic tool in medicine. leinco technologies uses the elisa as a quality control test to validate our antibodies and proteins. An elisa is used to detect the presence of an antibody or antigen in a sample. this tool is used heavily as a diagnostic tool in medicine but, is mainly used as a quality control test at leinco technologies. this protocol provides an initial set of conditions; however, further optimization may be required on an individual basis.

Sandwich Elisa Protocol Guide Leinco Technologies Sandwich elisa (as capture antibody with #57004 as hrp conjugated detection antibody). these are recommended concentrations end user should determine optimal concentrations for their applications.sandwich elisa protocol on next page. see specific product references below for more information. Learn how to perform a sandwich elisa from beginning to end. a sandwich elisa measures the levels of an antigen using two different antibodies (a capture and a detector antibody). the target antigen must contain at least two antigenic sites capable of binding to antibodies. monoclonal or polyclonal antibodies can be used as the capture and detection antibodies in sandwich elisa systems. 15 minutes approx. 1. collect blood samples in tubes with anti coagulant. anti coagulant should be diluted to a final concentration of 0.1 m. the anti coagulant prevents the blood from clotting. 2. spin down samples in a centrifuge and remove the pellet. centrifuge at 1,000 – 10,000 x g for 10 min at 4 °c. The volume per well should be the same as the capture antibody used in step 1. remove the blocking buffer and add the samples and standards. cover the plate and incubate for 1 hour at rt. remove the solution and wash the plate with 200 l per well wash buffer for 3 x 5 minutes on a shaking platform.