Cfda Se Cell Proliferation And Tracking Dye Stemcell Technologies

Cfda Se Cell Proliferation And Tracking Dye Stemcell Technologies Cfda se is frequently used in cell proliferation assays, as it is partitioned approximately equally between the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions. cfda se is also used for motility assays and in vivo cell tracking experiments. Incubate cells with the dye for 5 10 minutes in the dark at 37°c or room temperature (15 25°c). add an equal volume of culture medium containing 10% fetal bovine serum (fbs) and incubate for 5 minutes to quench the staining. pellet the cells by centrifugation and wash once with an equal volume of culture medium.

Cfda Se Cell Proliferation And Tracking Dye Stemcell Technologies Membrane permeable fluorescent probe for the detection of live neurons. resazurin (sodium salt) cell proliferation and viability dye. dapi (hydrochloride) dna labeling dye. aldhbr assay kit. for the detection of cd34 and aldhbr cells in human cord blood. carmine alum. dye for staining mouse mammary gland whole mounts. Cfda, se is a fixable cell permeant, fluorescein based tracer for very long term cell labeling. the dye is also available in our convenient, easy to use vybrant cfda se cell tracer kit (v12883). 5(6) cfda, se; cfse (5 (and 6) carboxyfluorescein diacetate, succinimidyl ester), mixed isomers. With cfda se (cfse) stock and storage: prepare cfda se at a stock concentration 1000 fold higher than the final usage concentration (for example, 2 mm if the final concentration is 2 um) in dry dmso. aliquot into single usage vials and store over desiccant at –20°c. cfda se will hydrolyze quickly at room temperature in the presence of water. Briefly, 50 µg carboxyfluorescein diacetate succinimidyl ester (cfda se) was reconstituted in 18 µl dmso (celltrace cfse cell proliferation kit, thermo fisher scientific #c34554) to create a 5.